Working mechanism of P-152
Phase I: Initiation
Research indicates that surfaces coated with P-15 promote cell attachment. However, the exact mechanism by which cell attachment is promoted is yet to be determined. Furthermore, P-15 promotes cell adhesion by providing a network of surface adhesion points for cell attachment. Additionally, studies have shown that cell spreading and the formation of lamellipodia and filopodia are stimulated by P-15.
Phase II: Activation
The presence of focal adhesions, which can be interpreted as anchor points between the cytoskeleton, plasma membrane and the surface adhesion sites with P-15, can be noted in case of the transition stable sites of cell-substrate adhesion. Experimental studies have shown cell activation and focal adhesion formation The experimental studies made use of different cell cultures and although P-15 promoted cell adhesion and activation, different pathways of integrin signalling were noted.
Phase III: Amplification
Furthermore, research indicates that P-15 promotes the attraction, attachment, proliferation and differentiation of cells. Enhanced expression of growth factor beta 1 (TGF-B1), bone morphogenetic protein 2 (BMP-2) and BMP-7 hours-days after cell attachment to P-15 coated surfaces was shown. The enhanced secretion of these cells may induce cell proliferation, attraction of cells towards cytokine and morphogen secreting cells as well as increased cell differentiation.